The clock component OsLUX regulates rice heading through recruiting OsELF3-1 and OsELF4s to repress Hd1 and Ghd7
Peng Xu,Yingxin Zhang,Xiaoxia Wen,Qinqin Yang,Ling Liu,Shulei Hao,Jiaxin Li，Zhaozhong Wu，Liaqat Shah，Amir Sohail，Qunen Liu，Lianping Sun，Yongbo Hong，Daibo Chen，Xihong Shen，Xiaodeng Zhan，Shihua Cheng*，Liyong Cao* and Weixun Wu*
Journal of Advanced Research
10.1016/j.jare.2022.08.001
Abstract
Introduction: Circadian clocks coordinate internal physiology and external environmental factors to regulate cereals flowering, which is critical for reproductive growth and optimal yield determination.
Objectives: In this study, we aimed to confirm the role of OsLUX in flowering time regulation in rice.
Further research illustrates how the OsELF4s–OsELF3-1–OsLUX complex directly regulates floweringrelated genes to mediate rice heading.
Methods: We identified a circadian gene OsLUX by the MutMap method. The transcription levels of
flowering-related genes were evaluated in WT and oslux mutants. OsLUX forms OsEC (OsELF4s–OsEL
F3-1–OsLUX) complex were supported by yeast two-hybrid, pull down, BiFC, and luciferase complementation assays (LCA). The EMSA, Chip-qPCR, luciferase luminescence images, and relative LUC activity